Molecular Biology Overview
The scientists at Marin Biologic have extensive experience in cloning and analyzing genes from both prokaryotic and eukaryotic cells. We will work with you to develop expression, production and analysis strategies and take into account your final use. With our experience in biochemistry, we can purify your protein to homogeneity using molecular biology and biochemistry approaches.
Marin Biologic scientists can also express your gene of interest. We are able to produce constructs in the system of your choice. From your expression construct we can further express the protein of interest and test it in other cell-based assays. If you are working with siRNA, we have experience in regulating mammalian genes using this technology.
We can create genomic or cDNA libraries for you from tissues or cell culture with expertise. Not only can we make your DNA library, we can clone your gene of interest for analysis in a customized bioassay.
If you already have your gene of interest subcloned we can perform the DNA isolation for you. If not, we are skilled in subcloning and can further perform your gene expression analysis or subclone your gene of interest into a mammalian expression vector. With our experience in cell culture, we can expand your cells and harvest your protein for purification (see Cell Biology and Biochemistry).
Our scientists are able to perform various reporter assays with your reporter constructs. We have experience in many different reporter assays such as CAT assays, luciferase assays and various other fluorescent or chemiluminescent reporter assays. We can always create a custom reporter assay for you.
- RNA Analysis
- RNA DNA Isolation
- Reporter Assay
- Gene Expression & Cloning
- Protein Production and Expression
- Northern Blot
- Dot Blot or Slot Blot Hybridization
- RT-PCR, Reverse-Transcriptase Polymerase Chain Reaction
- In situ Hybridization
- 5′, 3′ RACE
- RNase Protection Assay
- CAT Assays
- Luciferase Assays
- Fluorescence Assays
- Chemiluminescence Assays
- Custom Assays
- Restriction Endonuclease Digestion and Fragment Mapping
- DNA Subcloning – High Throughput
- cDNA Library and Genomic Library
- Site-Directed Mutagenesis
- Recombinant Protein Expression and Manufacture (Baculovirus, Bacteria, Yeast)