ELISA Assay Development | Monoclonal Antibody Development
ELISA (ENZYME LINKED IMMUNO-SORBANT ASSAY) technology can be used for preclinical, clinical PK studies, and detection of host anti-drug antibodies (ADA), Some purposes and methodologies include:
- GMP lot release assay
- Affinity (Kd) measurements
- Quantitation of cytokines or other proteins produced by cells or in the body
- Triggering of signal transduction as detected by phosphory-lated receptors, and other purposes
At Marin Biologic, we have:
- Employed optical, fluorescent, luminescent, time-resolved and radioimmune (RIA) detection methods using either monoclonal or polyclonal antibodies
- Utilized proprietary methods whenever necessary
- Removed interferences such as analyte binding to proteins and receptors or interfering host antibodies of various types
- Developed over 60 ELISAs, starting with producing our own monoclonal antibodies and polyclonal antibodies or screening commercial and sources
- Validated over 30 methods for GLP or GMP use
Purification and Characterization
- Affinity Chromatography
- Ion Exchange Chromatography, HPLC (see Biochemistry)
- Electrophoresis
- lg Class Identification and Affinity
- Epitope Mapping
- Hybridoma Production and Cloning
- Monoclonal Antibody Production
- Monoclonal Antibody Assay Development
- Monoclonal Antibody Manufacture
- Hollow Fiber Reactor
- Suspension Culture
- (mg to 100 g level)
- Monoclonal Purification (see Biochemistry)
- Peptides and Haptens
- Biotin, Fluorochrome
- Enzyme
- Radiolabel Conjugates
- Fab, F(ab’)2 Derivatives